crispr_therapy: 5-azacytidine was obtained from selleckchem (s1782) and hct116 cells were cultured in the presence of 5 um or 1:2000 dmso control. cells were plated in media containing 5-aza or dmso and harvested at 24 h and 7 days. media was refreshed with fresh 5-aza every 2nd day for the 7 day culture.